The 1932 comedy Gli uomini che mascalzoni (What scoundrels men are!) with a very young Vittorio De Sica open with a long tram chase.
If you never happened to see it, linked here you can find a few selected scenes from What scoundrels men are! (which is currently in the Public Domain). It’s a light comedy as befitted Italy in an age when Fascism had already been in power for a few years, but it is also packed with vintage images of Milan. Check out these images here or clicking on the photo.
The tram car in the sequence, a classic Carrelli 1928 car, is still running. Originally painted green, it is now orange-colored and serves on the line 23, circling the center.